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Journal: bioRxiv
Article Title: Metabolic engineering of Escherichia coli strains for the in vivo synthesis of GP-mediated oligosaccharides
doi: 10.64898/2026.02.23.707450
Figure Lengend Snippet: A. Chemical structure of β-1,2-mannobiose, showing the anomeric protons of the reducing (ManA) and the non-reducing (ManB) mannosyl moieties (highlighted in blue), corresponding to the signals observed in the NMR spectra in panel B. B. Selected regions of the 1D 1 H NMR spectra of β-1,2-mannobiose corresponding to the anomeric proton signals. C. HPAEC-PAD chromatograms showing that the β-1,2-mannobiose produced in the culture supernatant co-eluted with a β-1,2-mannobiose standard. Bright green lines, β-1,2-mannobiose standard; red, culture supernatant of PFKA1-Teth514_1788; blue, culture supernatant of PFKA1-control .
Article Snippet:
Techniques: Produced, Control